Understanding Metagenomics
Metagenomics is an interdisciplinary field of genetics that includes studying environmental samples (such as soil, food, and water) using genetic techniques. With metagenomics, we can study and investigate microbes in a single experiment which is impossible with conventional microbiology methods.
Metagenomics has been proven as a powerful tool that is potent enough to penetrate different fields, to solve various problems. It is used widely in ecology, environment conservation, infectious disease diagnosis, Environmental remediation, Biotechnology, and agriculture.
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In this workshop, we shall be demonstrating various methods used in exploring the metagenomics study of water, soil, food, and clinical microbial samples.
Abstract
Antibiotic resistance genes (ARGs) and antimicrobial resistance elements (AMR) are novel environmental contaminants that pose a significant risk to human health globally. Freshwater contains a variety of microorganisms that might affect human health; its quality must be assessed before use. However, the dynamics of mobile genetic elements (MGEs) and ARG propagation in freshwater have rarely been studied in Singapore. Therefore, this study used metagenomics to compare diversity, virulence factor composition, and ARG and MGE co-occurrence with bacterial communities in paired (n = 8) environmental freshwater samples. KneadData, FMAP, and Kraken2 were used for bioinformatics analysis and R (v4.1.1) for statistical analysis. Sequence reads with a total of 9043 species were taxonomically classified into 66 phyla, 130 classes, 261 orders, 584 families, and 2477 genera. Proteobacteria, Bacteroidetes, Actinobacteria, and Firmicutes were found in the Phyla in all samples. Analysis of QIIME output by PICRUSt and ß-diversity showed unique clusters and functional microbial community structures...
Recent Research Publication on Freshwater Metagenomics by our Students
Who this workshop is for?
Those Interested in Research and Publication
If you want to explore the microbial community, effectively analyze your data, interpret results and come up with key insights for research publications, this workshop is for you.
Life Science Researchers and Students
All Undergraduates, Masters, Ph.D., Researchers, and Faculties in life science fields which include medical sciences, agricultural sciences, biological sciences, biochemistry, etc.
Medical Practitionals
If you have a key interest in infectious disease or diagnosis, epidemiology, outbreak detection, pathogenic surveillance, etc, then this workshop is for you.
MODULES TO BE COVERED
1
Introduction to Metagenomics
Overview of comparative genomics principles and techniques
2
Genome Assembly Techniques
Genome assembly methods and quality control measures for preprocessing raw sequencing data.
3
Comprehensive Metagenomics Analysis
​Functional annotation methods for identifying and characterizing gene functions in the samples
4
Subsystem functional categorization
Performing functional categorization of quarry samples including subsystems and genes associated with them.
5
Taxonomic Classification
Identify the community of microbial species or strains in the sample: "Comparing genomic data to classify and quantify microbial taxa".
6
Comparative AMR Gene Analysis
Examine the prevalence of antibiotic-resistant genes across samples, and their association with specific antibiotic classes.
7
Virulence Factor Identification
Map sequencing data to virulence gene databases (e.g., VFDB) to identify virulence factors and their associated genes.
7
Drug Targets
Predict potential drug targets using annotated genes and model protein structures to aid drug discovery and therapeutic design.
7
Visualization and Interpretation
Create comprehensive visualizations, including heatmaps, phylogenetic trees, and comparative plots for AMR and virulence factor profiling.
TESTIMONIALS
Dr. Maryam Idrees Malik
Before starting the program, I was a bit apprehensive, as I had previously been taken advantage of by some online scam. But now, I consider myself fortunate that I had stumbled upon this organisation. Kudos team genomac hub! I honour your intentions to create a global impact by helping researchers. Looking forward to learning and growth & thank you for sharing the lifetime activation key of geneious software.
Dr. Noman Sohail
During my time with Genomac Hub, I was offered online classes, clear guidance, and a structured map to proceed with the work, outlining the amazing results. The negotiations were satisfactory overall. I must rate the service 8/10
Blessing O
Attending Genomac Hub workshop helped me navigate the fundamentals of Bioinformatics. It provides a solid foundation for me to move on in my career. The CEO, Oluwaseyi, is very knowledgeable about the course. He is adept at keeping the class engaged and making everyone understand. I recommend Genomac Hub courses and workshops to anyone in any part of the globe.
Nikita Singh
Interesting, understandable, keeps the target audience engaged till the end. The concepts and protocols are explained in a lucid way.