Shotgun-Metagenomics | GENOMAC INSTITUTE

Shotgun Metagenomics Analysis

Workflow Analysis (Comparative Approach)

Stage 1

Sequence reads processing and genome assembly
Retrieval of the genome sequence of other strains for comparative analysis
General genome features (tabular)
Comprehensive metagenome analysis (annotation/gene prediction; hypothetical and functional proteins, enzyme commission, genome features, protein features)
Subsystem functional categorization of query sample(s)
Genome sequence properties (tabular) of query sample and other comparative strains

Stage 2

Taxonomic Classification
Plasflow Analysis (chromosomal, plasmid and unclassified sequences)
Antimicrobial Resistance Profiling
Comparative AMR genes (prevalence of antibiotic-resistant genes per samples and classes of antibiotics, resistance gene distribution, frequency comparison, etc.)
Comparative resistant mechanism
Shared resistance genes between strains
The pattern of antimicrobial resistance in a region

Stage 3

Virulence Factors
Comparative virulence factors and genes between strains
Shared specific genes
Drug targets and Protein Structures (optional)
Mobile Gene Elements/Transporters

Stage 4

Computing data and visualization of the results: (Statistical visualization which includes heatmaps, tabular, Venn diagram, bar charts, clusters, etc.)
Report (Materials and Method, Results and References)

The workflow as we have shown you entails the steps of bioinformatics analysis to be carried out for your data and each is unique to the workflow designed for the final outcome.

The analysis will be conducted providing your desired visualization for each major steps.

The following will be provided after the complete job is done.

Assembly genome sequence
A detailed draft of a report containing the materials and methods, results, and references
Visualizations (figures)
Technical support: A live zoom session will be held to explain and discuss the results with you.

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You can book a free session with us to discuss what you want to do, your research project, and show you a demo report. We promise to deliver accurate and trusted work done.

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